Downloaded OS X update and now Roon won't play

Not necessarily. Although it probably won’t hurt to reinstall the app, I’m not sure that would be the issue.

Maybe tell us a bit about your setup. Where is you music located? Is it on a hard drive connected to the computer or is it on a NAS? See this thread.

Also, it would be a good idea to quit the app, and restart the computer and router, etc. And see if that helps.

I update my Mac OS X a couple of days ago and everything went fine.

Cheers, Greg