I found that I could get it all working nicely but I ran into a problem after running AO Service Tool P, which prepares prior to the AO program itself.
I have since installed the Essentials Experience Role using Server Management (I am using 2012R2 Essentials) and the Media pack (see my cry for help here) which enables streaming but have not yet shifted databases for another attempt.
It does seem odd that I can get a functional RoonServer before the Preparation tool without the Experience Role and the Media Pack, so I am not terribly confident that those things are the necessary components that will fix any issue arising from using the Preparation tool.
Keep reporting back, lets see how far we can get !